Thromboangiitis Obliterans (TAO) Model

Greentech provides validated rat thromboangiitis obliterans (TAO) models and comprehensive in vivo pharmacology and pharmacodynamic services to support the discovery and preclinical development of therapies for peripheral vascular diseases. Backed by an experienced cardiovascular research team and advanced preclinical research platforms, we deliver high-quality efficacy studies that support both domestic and international IND programs.

Our Rat Thromboangiitis Obliterans (TAO) Model

Greentech has established a sodium laurate-induced rat TAO model through femoral artery injection. This well-characterized model reproduces key pathological features of thromboangiitis obliterans and is widely used for preclinical efficacy evaluation of candidate therapeutics.

Induction: Intra-femoral arterial injection of sodium laurate following temporary arterial occlusion.

Animals: Male rats

Characteristics: Following sodium laurate administration, animals develop progressive ischemic changes in the affected limb, including:

      1) Reduced skin temperature

      2) Pallor followed by cyanosis

      3) Decreased or absent arterial pulsation

      4) Toe flexion and discoloration

      5) Ischemic necrosis of the digits

      6) Limping or impaired limb function

These pathological and functional changes closely resemble the clinical manifestations of thromboangiitis obliterans.

Positive Control Drugs: commonly used positive controls include 

      1) Tongsaimai Tablets

      2) Alprostadil

      3) Clopidogrel sulfate

Study Endpoints

Greentech offers comprehensive efficacy assessment, including

      1) Limb ischemia severity scoring

      2) Skin blood perfusion measurement

      3) Body weight change

      4) Additional customized pharmacodynamic endpoints upon request

Representative Studies

Figure 1. Representative images and limb ischemia scores in the sodium laurate-induced rat TAO model.

Figure 2. Reduction in skin blood perfusion in the sodium laurate-induced rat TAO model.

References

1.Feng X, Wang Y, Zhu Y, et al. Improvement of vascular inflammatory injury in a rat model of thromboangiitis obliterans by Liangxue Heying Formula via the JAK2/STAT3 signaling pathway. Chinese Traditional Patent Medicine. 2024;46(9):2915–2922.

Inquiries

Request a quote now, or email us at BD@greentech-bio.com to inquire about our services or obtain a quote for your project.