DMPK & Non-GLP Toxicology

In Vitro ADME

The evaluation of pharmacological properties including absorption, distribution, metabolism, and excretion (ADME) is critical for screening novel drug candidates. Greentech has rich experience in pharmacokinetics, including in vitro ADME, in vivo PK and small molecule bioanalysis. At lead generation and optimization stages, we provide in vitro ADME screening and validated assays.

ADME Screening Assays

Permeability tests: PAMPA,Caco-2,MDR1-MDCK

Metabolic stability tests: liver microsomes from human and preclinical species

CYP450 enzyme inhibition tests: single or mixed substrates, estimation of percentage inhibition or IC50

Plasma protein binding tests: plasma isolated from human and preclinical species

Whole blood/plasma distribution tests: multiple species

Validated Assays

CYP450 enzyme inhibition tests: 8 CYP subtypes, estimation of IC50, Ki, Kinact, reversible/irreversible inhibition, time-dependent inhibition (TDI) of CYP

CYP450 enzyme induction tests: frozen human hepatocytes, estimation of enzyme activity and mRNA level

CYP phenotype analysis: multiple species

Plasma protein binding tests: equilibrium dialysis, ultrafiltration and ultracentrifugation

Case Study

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