Models of Renal Failure

Greentech provides validated rodent models of 5/6 nephrectomy induced chronic kidney failure and models of partial ligation of the abdominal aorta induced acute kidney failure, delivering robust data reporting for your project.

Our Animal Models of Renal Failure

1. Models of chronic kidney disease – 5/6 nephrectomy model

Chronic kidney disease (CKD) is a worldwide health issue, affecting 13% of the global population. Subtotal 5/6 nephrectomy (5/6 Nx, or PNx) is the most frequently established model of CKD.PNx is achieved by ablation of 5/6 renal parenchyma, causing progressive renal failure.

Model characteristics: mesangial cell proliferation, mesangial matrix accumulation, glomerular hypertrophy and sclerosis, and interstitial fibrosis, consistent with the process of human renal fibrosis.

2. Models of acute renal failure – partial ligation of the abdominal aorta

Acute renal failure (ARF) in humans typically results from hypoperfusion, and therefore, a hypoperfusion model of ischemic ARF more closely simulates clinical ARF. In this model, temporary partial aortic ligation (PAL) is created after uninephrectomy, leading to renal tubule epithelial cell injury and finally ARF.

Model characteristics: Partial aortic ligation induced renal failure model exhibits renal enlargement, diffuse swelling and partial necrosis of renal tubular epithelial cells, renal interstitial edema. The serum creatinine level is higher than a normal level at 24h, 48h and 72h after surgery.

Animal Species

Mice, rats

Clinical Assessment

Clinical observation, body weight, residual kidney weight

Blood biochemistry: BUN, Scr, etc.

Urine routine and 24h urine protein quantification

Serum immunoassay: TGF-β1, FN (ELISA)

Renal histopathology: HE, PAS, Masson staining

Immunohistochemistry: TGF-β1、FN、α-SMA

Real-time quantitative PCR

Case Study

1. SD rat model of 5/6 nephrectomy (5/6 Nx)

2. Partial aortic ligation induced acute renal failure model

Inquiries

Request a quote now, or email us at BD@greentech-bio.com to inquire about our services or obtain a quote for your project.

References

1. Zager RA. Partial aortic ligation: a hypoperfusion model of ischemic acute renal failure and a comparison with renal artery occlusion. J Lab Clin Med. 1987 Oct;110(4):396-405.

2. Philipp Kalk, Michael Godes, Katharina Relle, Christiane Rothkegel, Andreas Hucke,Johannes-Peter Stasch ,Berthold Hocher. NO-independent activation of soluble guanylate cyclase prevents disease progression in rats with 5/6 nephrectomy.British Journal of Pharmacology (2006) 148, 853–859.

3.  Gava A L , et al. Effects of 5/6 nephrectomy on renal function and blood pressure in mice. International Journal of Physiology Pathophysiology & Pharmacology, 2012, 4(3):167-73.