Greentech provides validated rodent models of 5/6 nephrectomy induced chronic kidney failure and models of partial ligation of the abdominal aorta induced acute kidney failure, delivering robust data reporting for your project.
Chronic kidney disease (CKD) is a worldwide health issue, affecting 13% of the global population. Subtotal 5/6 nephrectomy (5/6 Nx, or PNx) is the most frequently established model of CKD.PNx is achieved by ablation of 5/6 renal parenchyma, causing progressive renal failure.
Model characteristics: mesangial cell proliferation, mesangial matrix accumulation, glomerular hypertrophy and sclerosis, and interstitial fibrosis, consistent with the process of human renal fibrosis.
Acute renal failure (ARF) in humans typically results from hypoperfusion, and therefore, a hypoperfusion model of ischemic ARF more closely simulates clinical ARF. In this model, temporary partial aortic ligation (PAL) is created after uninephrectomy, leading to renal tubule epithelial cell injury and finally ARF.
Model characteristics: Partial aortic ligation induced renal failure model exhibits renal enlargement, diffuse swelling and partial necrosis of renal tubular epithelial cells, renal interstitial edema. The serum creatinine level is higher than a normal level at 24h, 48h and 72h after surgery.
Clinical observation, body weight, residual kidney weight
Blood biochemistry: BUN, Scr, etc.
Urine routine and 24h urine protein quantification
Serum immunoassay: TGF-β1, FN (ELISA)
Renal histopathology: HE, PAS, Masson staining
Real-time quantitative PCR
1. SD rat model of 5/6 nephrectomy (5/6 Nx)
2. Partial aortic ligation induced acute renal failure model
1. Zager RA. Partial aortic ligation: a hypoperfusion model of ischemic acute renal failure and a comparison with renal artery occlusion. J Lab Clin Med. 1987 Oct;110(4):396-405.
2. Philipp Kalk, Michael Godes, Katharina Relle, Christiane Rothkegel, Andreas Hucke,Johannes-Peter Stasch ,Berthold Hocher. NO-independent activation of soluble guanylate cyclase prevents disease progression in rats with 5/6 nephrectomy.British Journal of Pharmacology (2006) 148, 853–859.
3. Gava A L , et al. Effects of 5/6 nephrectomy on renal function and blood pressure in mice. International Journal of Physiology Pathophysiology & Pharmacology, 2012, 4(3):167-73.