Greentech Bioscience offers several validated animal models of osteoarthritis pain for studying diseases mechanisms and assessing treatments against osteoarthritis pain. All studies are performed at our AAALAC accredited animal facilities and laboratories at Greentech.
Osteoarthritis (OA) is one of the most common joint disease, causing much pain. Although osteoarthritis pain is the cardinal symptom of OA and directly affects function and quality of life, the pathogenesis mechanisms are not clear and current treatments are limited by efficacy and adverse effects. Greentech offers several well-established animal models of OA pain to study the disease mechanisms and drug efficacy testing. We also offer several validated animal models of osteoarthritis.
Monosodium iodoacetate (MIA), a cysteine peptidase inhibitor, is the most commonly used agent for developing the OA pain model. Intra-articular injection of MIA in rodents induces cartilage degeneration by suppression of chondrocyte metabolism, leading to osteoarthritis. The MIA model has been the model of choice in the pain field due to its demonstration of long-lasting hyperalgesia and weight bearing asymmetry. The MIA model is an easy and reproducible experimental model for assessing OA pain or other symptoms of OA. We also provide MIA induced osteoarthritis model.
Surgically induced animal models can reflect posttraumatic human osteoarthritis, which are widely used to assess OA pain. Common surgically induced OA pain models include anterior cruciate ligament transection (ACLT), medial meniscus tear (MMT) and so on. ACLT simulates human anterior cruciate ligament rupture and reflects the pathological process and pain of osteoarthritis. MMT mimics human meniscus damage, forming cartilage defects and osteophytes and causing joint pain. Both models cause joint instability, including cartilage degeneration, bone remodeling, pain and other OA lesions, suitable for posttraumatic osteoarthritis pain research.
Mice, rats, rabbits
(1) Body weight, clinical observations
(2) Behavioral tests: mechanical hyperalgesia, thermal hyperalgesia, gait analysis, incapacitance test
(3) Optional indexes: joint histopathology and micro-CT
MIA induced rat model of osteoarthritis pain model
1. Hong JI,Park IY,Kim HA.Understanding the Molecular Mechanisms Underlying the Pathogenesis of Arthritis Pain Using Animal Models.[J].Int J Mol Sci,2020,2.
2. Alves-Simões M.Rodent models of knee osteoarthritis for pain research.[J].Osteoarthritis Cartilage,2022,6:802-814.