Models of Pulmonary Arterial Hypertension

Greentech Bioscience offers monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH) rat model for efficient drug efficacy testing. Based on our experienced pharmacology and pharmacodynamics team and validated animal model library, we ensure reliable and reproducible data.

Rat Model of Pulmonary Arterial Hypertension

Monocrotaline-treated rats are frequently used in the preclinical studies of anti-PAH drugs. Monocrotaline cause pulmonary vascular remodeling and increased vascular resistance, ultimately leading to heart failure.

Induction: pulmonary vascular endothelium can be selectively damaged by intraperitoneal or subcutaneous injection of MCT, leading to PAH after 3~4 weeks.

Species: rats

Disease characteristics: monocrotaline-induced PAH in rats are similar with human PAH in hemodynamics and histopathology. MCT that causes endothelial dysfunction can mimic human PAH with inflammatory conditions, but hardly PAH associated with severe angiogenesis.

Study Endpoints

      1) Right ventricular systolic pressure (RVSP)

      2) Right ventricular hypertrophy index (RVHI)

      3) Pulmonary vascular remodeling

Representative Studies

Figure 1. Right ventricular systolic pressure (RVSP) and right ventricular hypertrophy parameters, including RV free wall thickness, RV/(LV+S), and RV/BW, in the rat pulmonary arterial hypertension (PAH) model.

Figure 2. RV free wall thickness in the rat pulmonary arterial hypertension (PAH) model.

References

1. Kimura S, et al. Nanoparticle-mediated delivery of nuclear factor kappaB decoy into lungs ameliorates monocrotaline-induced pulmonary arterial hypertension. Hypertension. 2009 May;53(5):877-83.

2. Peiran Yang, et al. A novel cyclic biased agonist of the apelin receptor, MM07, is disease modifying in the rat monocrotaline model of pulmonary arterial hypertension. Br J Pharmacol.2019 May;176(9):1206-1221.

Inquiries

Request a quote now, or email us at BD@greentech-bio.com to inquire about our services or obtain a quote for your project.